keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Deoxycholic acid sodium salt (Sodium deoxycholate) 是 G 蛋白偶联胆汁酸受体TGR5的激活剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 282 | 现货 | ||
100 mg | ¥ 387 | 现货 | ||
500 mg | ¥ 541 | 现货 |
产品描述 | Deoxycholic acid sodium salt (Sodium deoxycholate) can activate the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity. |
体外活性 | Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA) are the common ingredients of duodenal reflux that act synergistically in many physiological and pathological processes. The cells are repeatedly exposed to 100 μM CDCA and Deoxycholic acid at pH 5.5 for up to 120 min. To simulate chronic local recurrent disease in vitro, the gastric cancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 μM Deoxycholic acid and CDCA. An untreated log-growth MGC803 cell line is generated to be used as a control in normal pH media. After daily 10 min exposure to the acidified bile acids for 60 weeks, MGC803-resistant cells are able to survive and proliferate after 120 min exposure [2]. |
细胞实验 | MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 μM (B) with culture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks[2]. |
别名 | 脱氧胆酸钠, Sodium deoxycholate, Sodium Desoxycholate |
分子量 | 414.56 |
分子式 | C24H39NaO4 |
CAS No. | 302-95-4 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 330 mg/mL
DMSO: 12.5 mg/mL (30.15 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4122 mL | 12.061 mL | 24.122 mL | 60.3049 mL |
5 mM | 0.4824 mL | 2.4122 mL | 4.8244 mL | 12.061 mL | |
10 mM | 0.2412 mL | 1.2061 mL | 2.4122 mL | 6.0305 mL | |
20 mM | 0.1206 mL | 0.603 mL | 1.2061 mL | 3.0152 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Deoxycholic acid sodium salt 302-95-4 GPCR/G Protein Metabolism Endogenous Metabolite GPCR19 Inhibitor G-protein coupled receptor 19 bile acid TGR5 Deoxycholic acid G protein-coupled bile acid receptor 脱氧胆酸钠 GPBAR1 Deoxycholic acid sodium G protein-coupled Bile Acid Receptor 1 inhibit Sodium deoxycholate Sodium Desoxycholate inhibitor